The Patricia A. Berardi
Gallbladder Cancer Research Program
Literature, news and videos pertinent to
The Patricia A. Berardi Gallbladder Cancer Research Program
The Operating Room of the Future – Video regarding the pathbreaking work of InSightec
based in Tirat Carmel, Israel, www.insightec.com
- Tiny capsule effectively kills cancer cells
- Life-Saving DNA Test Overlooked in Rise of Colon Cancer
- Serial analysis of gene expression identifies connective tissue growth factor expression as a prognostic biomarker in gallbladder cancer.
Clin Cancer Res. 2008 May 1;14(9):2631-8.
Alvarez H, Corvalan A, Roa JC, Argani P, Murillo F, Edwards J, Beaty R, Feldmann G, Hong SM, Mullendore M, Roa I, Ibañez L, Pimentel F, Diaz A, Riggins GJ, Maitra A.
Abstract
Background:
Gallbladder cancer (GBC) is an uncommon neoplasm in the United States, but one with high mortality rates. This malignancy remains largely understudied at the molecular level such that few targeted therapies or predictive biomarkers exist.
Experimental Design:
We built the first series of serial analysis of gene expression (SAGE) libraries from GBC and nonneoplastic gallbladder mucosa, composed of 21-bp long-SAGE tags. SAGE libraries were generated from three stage-matched GBC patients (representing Hispanic/Latino, Native American, and Caucasian ethnicities, respectively) and one histologically alithiasic gallbladder. Real-time quantitative PCR was done on microdissected epithelium from five matched GBC and corresponding nonneoplastic gallbladder mucosa. Immunohistochemical analysis was done on a panel of 182 archival GBC in high-throughput tissue microarray format.
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